628634, 1992. No other enteropathogenic bacteria, including Vibrio cholerae, Salmonella, Vibrio parahaemolyticus, Proteus vulgaris or Staphylococcus aureus, were detected from those 8 samples. This bacterium can attach to biotic/abiotic surfaces to form biofilms which contribute to seafood quality degradation and shelf-life reduction. 9, no. Clinical Microbiology and Infection, 2006. Examine the KIA tube on the right in the image provided and determine which of the following organisms produces the biochemical reaction seen. doi: 10.1371/journal.pone.0096001. Clipboard, Search History, and several other advanced features are temporarily unavailable. Indian J Med Microbiol. Phylogenetic tree was constructed with MEGA 4.0. 262264, 2013. Compared with the 25C group, only 50% hemolysis occurred after 72 hours. Infect Drug Resist. Shewanella spp. The biochemical profiles showed that all of the strains were unable to utilize some carbohydrates, but produced hydrogen sulfide (H 2 S). 6, pp. Time course transcriptome changes in Shewanella algae in response to salt stress. S. algae has a G + C content of 52-54%[6]. The reaction mixture was placed on ice to terminate the reaction and then desalted with . Shewanella is a saprophytic gram negative bacillus which is naturally associated with marine and aquatic habitat. 5, pp. 50, no. Shewanella algae - microbewiki - Kenyon College Salmonellae: Classification, Biochemical Reaction and Features The one-pot CRUDE (Conversion of Raw and Untreated Disposal into Ethanol) process was developed for simultaneous hydrolysis and fermentation of unprocessed food waste into ethanol using thermophilic (growing at 65C) anaerobic bacteria. A functional description of CymA, an electron-transfer hub supporting 350, no. Acetone-h 6 or -d 6 + OH Reaction Products: Evidence for Heterogeneous Formation of Acetic Acid in a Simulation Chamber. 5, pp. -, Differentiation of Shewanella putrefaciens and Shewanella alga on the basis of whole-cell protein profiles, ribotyping, phenotypic characterization, and 16S rRNA gene sequence analysis. Frontiers | Insights on nitrate respiration by Shewanella International journal of molecular sciences, 2019, 20(9): 2143. In this chapter we will discuss the engineering of cyclic hydroxamate siderophores by various biochemical approaches based on the example of Shewanella algae. 11181123, 2002. In Shewanella, the mechanism behind the regulation of the intracellular concentration of this effector molecule is unknown. It is used as many things from a bioremediate of chlorinated compounds to a radionuclide and a biocatalyst. It has the ability to produce hydrogen sulfide and reduce Fe(III), making it very corrosive of metal surfaces[12]. Although these bacilli were thought to be rarely pathogenic, there has been an increasing number of reports of them being implicated in a wide variety of clinically significant infections. The extracted DNA was stored at 20C until processing. Kwong-Chung Tung revised the manuscript. Shewanella - an overview | ScienceDirect Topics They have the capacity to also live in low temperature waters[5]. government site. HHS Vulnerability Disclosure, Help Domain: Bacteria 36, no. PDF Influence of acetate and C02 on the TMAO-reduction reaction by Electron microscopic analyses revealed that iron promoted Te(IV) reduction led to form exclusively extracellular crystalline Te(0) nanorods, as compared to the mostly intracellular . 9, pp. The present study investigated . The reduction of tellurite (Te(IV)) by dissimilatory metal reducing bacterium, Shewanella oneidensis MR-1, was promoted in the presence of Fe(III) in comparison with Te(IV) bioreduction in the absence of Fe(III). We hope to increase the awareness among care providers on the same. and transmitted securely. Bacteria from the genus Shewanella are inhabitants of marine and freshwater ecosystems, recognized fish spoilage bacteria, but less known as fish disease agents. 4, pp. From these, we may infer S. algae adapt to environmental changes via different biosynthetic pathways. The results showed that S. algae is endemic in Asia. . S. algae is adapted to a wide range of temperatures (4C, 25C, 37C, and 42C) and salinity. Both components of the course are equally important in providing knowledge and actual laboratory experience to the participant. This finding is consistent with a number of studies conducted in areas with warm climates, largely in Asia [9, 41, 42]. Reaction mixtures were incubated in an Eppendorf of Perkin-Elmerk GeneAmp 9600 PCR system. Volume 11: 347-352. Reference ranges for hematologic and serum biochemical values in llamas (Lama glama) Slime mold beetles of the genus Anisotoma (Leiodidae): classification and evolution To be young, gifted, black and female: a meditation on the cultural politics at play in representations of Venus and Serena Williams T. J. DiChristina, C. M. Moore, and C. A. Haller, Dissimilatory Fe(III) and Mn(IV) reduction by Shewanella putrefaciens requires ferE, a homolog of the pulE (gspE) type II protein secretion gene, Journal of Bacteriology, vol. H. Larsen, Halophilic and halotolerant microorganisms-an overview and historical perspective, FEMS Microbiology Letters, vol. The authors have declared that no competing interests exist. They have been attributed to a wide array of disease in humans, such as ear infections and open wound infections[2]. 4, p. 428, 2011. Methods: M. Bally and J. Garrabou, Thermodependent bacterial pathogens and mass mortalities in temperate benthic communities: a new case of emerging disease linked to climate change, Global Change Biology, vol. Shewanella putrefaciens infective endocarditis | The Brazilian Journal B) Postsurgical. We also examined the biochemical and physiological changes in dinoflagellates exposed to the algicide. Page authored by Hannah Merrill and Deonte Swift, students of Prof. Jay Lennon at Michigan State University. H. M. Holt, P. Sgaard, and B. Gahrn-Hansen, Ear infections with Shewanella alga: a bacteriologic, clinical and epidemiologic study of 67 cases, Clinical Microbiology and Infection, vol. B. F. Vogel, H. M. Holt, P. Gerner-Smidt, A. Bundvad, P. Sgaard, and L. Gram, Homogeneity of danish environmental and clinical isolates of Shewanella algae, Applied and Environmental Microbiology, vol. S. algae is important in the cycling of organic material and the bioremediation of halogenated organic pollutants[1]. -. The data used to support the findings of this study are currently under embargo while the research findings are published. We found no significant difference between the two group ( and ). 11, pp. Characterization of putative pathogenic Shewanella algae isolated from The mussels were confirmed to be related to Crassostrea angulata, Meretrix lusoria, Perna viridis, Geloina erosa, and Haliotis diversicolor. Voges-Proskauer-positive. K. K. Sharma and U. Kalawat, Emerging infections: Shewanellaa series of five cases, Journal of Laboratory Physicians, vol. 38963900, 1999. e119e124, 2008. International journal of systematic bacteriology, Genetic and phenotypic studies on the strains biochemically identified as Shewanella putrefaciens, which had a G+C content ranging from 52 to 54 mol% were conducted. (PDF) Bioprospeco da Chromobacterium violaceum para a biorremediao The Biochemistry of Dissimilatory Ferric Iron and - ResearchGate 87, no. The findings in these 28 cases confirm the fact that S. putrefaciens can invade the bloodstream; however, the presence of concurrent bacteremia makes it difficult to determine the pathogenic role of this organism in septicemia. Most Shewanella alga organisms (Gilardi biovar 2; Centers for Disease Control and Prevention [CDC] biotype 2) originated from clinical material (92%), failed to produce acid from carbohydrates other than D-ribose, and were biochemically and enzymatically fairly homogeneous. L. Gram and H. H. Huss, Microbiological spoilage of fish and fish products, International Journal of Food Microbiology, vol. 894901, 2007. 11921194, 2009. Epub 2017 Mar 17. Y. J. Tang, A. L. Meadows, J. Kirby, and J. D. Keasling, Anaerobic central metabolic pathways in Shewanella oneidensis MR-1 reinterpreted in the light of isotopic metabolite labeling, Journal of Bacteriology, vol. J.-H. Lee and J. Lee, Indole as an intercellular signal in microbial communities, FEMS Microbiology Reviews, vol. Family: Shewanellaceae and transmitted securely. The growth curves of S. algae under different temperature are shown in Figure 2. J. J. DeFrank, W. T. Beaudry, T.-C. Cheng, S. P. Harvey, A. N. Stroup, and L. L. Szafraniec, Screening of halophilic bacteria and Alteromonas species for organophosphorus hydrolyzing enzyme activity, Chemico-Biological Interactions, vol. Shewanella algae and Shewanella putrefaciens: clinical and microbiological characteristics. The mechanism of S. putrefaciens biofilm formation is not yet described. Unable to load your collection due to an error, Unable to load your delegates due to an error. 18, 2002. Diversity of Shewanella population in fish Sparus aurata harvested in the Aegean Sea. The .gov means its official. Case Report: Shewanella Algae Pneumonia and Bacteremia in an Elderly Male Living at a Long-Term Care Facility. Shewanella is a ubiquitous organism that has been isolated from, Foodborne pathogens are the main threat and cause of food poisoning. Journal of Invertebrate Pathology, 2009. 2022 Apr 8;15:1645-1650. doi: 10.2147/IDR.S357181. The major characteristics in all S. algae isolates in this study include the ability to exert a strong hemolytic effect: an inability to utilize carbohydrates, although a few isolates were able to use maltose from some water samples. Diagnostic microbiology and infectious disease. 21892199, 1997. Left lower extremity can be visualized in this figure. 2005 May;11(5):347-52. doi: 10.1111/j.1469-0691.2005.01108.x. A. Beleneva, T. Y. Magarlamov, M. G. Eliseikina, and N. V. Zhukova, Biochemical and pathogenic properties of the natural isolate of Shewanella algae from peter the great bay, Sea of Japan, Journal of Invertebrate Pathology, vol. Orange-yellow or pink colonies on marine agar (BD) were identified as Gram-negative by Gram staining. Background: Shewanella spp. J Appl Microbiol. Risk factors associated with S. algae infections include chronic skin ulcer, chronic liver disease, and immune system disorders [57]. H. Sugita, K. Sugiyama, and S. Itoi, Culturable bacterial flora in the intestinal tract of Japanese Pufferfish Takifugu rubripes, Aquaculture Science, vol. 141148, 1993. 960963, 1956. Table 4 shows a list of results for S. algae infections in aquaculture animals from 1999 to 2017 in different countries [15, 16, 2840]. Nath R, Saikia L, Choudhury G, Das PP. The .gov means its official. [8] Myung, D.S., et al. 4, pp. Biochemical function and ecological significance of novel bacterial lipids in . Characterization of an Algicidal Agent Produced by the Bacterium Shewanella The mammal tion states ranging from +V to III, generating many inorganic SO catalyzes the last reaction in the oxidative degradation of sul- molecules that comprise the geochemical cycle of nitrogen (N- fur containing amino acids like cysteine and methionine, and plays cycle) [53-56]. 2014 Jun;15(3):336-8. doi: 10.1089/sur.2012.208. Biochemical Engineering Journal, 2012. 2005 Apr;35(4):186-91. doi: 10.1016/j.medmal.2005.03.008. Researcher Profile. The marine gamma-proteobacterium S. algae produces three different cyclic hydroxamate siderophores as metabolites via a single biosynthetic gene cluster and one of them is an important . K. A. Weber, L. A. Achenbach, and J. D. Coates, Microorganisms pumping iron: anaerobic microbial iron oxidation and reduction, Nature Reviews Microbiology, vol. 20, no. H. M. Holt, B. Gahrn-Hansen, and B. Bruun, Shewanella algae and Shewanella putrefaciens: clinical and microbiological characteristics, Clinical Microbiology and Infection, vol. Fonnesbech Vogel B, Venkateswaran K, Satomi M, Gram L. Appl Environ Microbiol. The moles percent G+C of the type. Liu, Z. Y. Shi, C. F. Lin et al., Shewanella infection of snake bites: a twelve-year retrospective study, Clinics, vol. identified by the biochemical system, more characteristics were analyzed using auxiliary biochemical, growth, hemolytic and drug-resistance tests. 181186, 2001. Tests also showed hydrolysis of gelatin, and decarboxylation of ornithine[6]. Understanding of both the biochemical and ecological features of . Temperature is a key parameter in the pathogenicity of S. algae as it appears to induce hemolysis at 25C and 37C. 4, pp. (PDF) Biochemical Characterization of A Novel - ResearchGate 1, pp. 477484, 2013. Identification of Shewanella baltica as the most important H2S-producing species during iced storage of Danish marine fish. (HEPES)-NaOH (pH 7.0), and 0.12 mM dithiothreitol was incubated at 20C for 10 min. Positive reactions in S. algae strains were seen for H2S (from sodium thiosulfate) and cytochrome oxidase (oxidase test) and gelatinase (gelatin); however, negative results were found for indole production (tryptophan) and carbohydrates utilization, including arabinose, mannose, mannitol, adipic acid, and phenylacetic acid (Table 3). 101, no. Furthermore, the seasonal growth and infection rate of S. algae peak during the summer. The ability of S. oneidensis to reduce oxidized metals or nitrate effectively has been identified as an important intrinsic activity of Shewanella species [5962]. Jonathan Eisen - Professor - University of California, Davis - LinkedIn 1, pp. The phenomenon suggests potential extensive water contamination which warrants continuous surveillance. In general, S. algae can be considered an opportunistic pathogen in humans exposed to a marine environment when it infects people via an existing soft tissue ulcer [1522]. 3, pp. 7, pp. 97, no. 10171026, 2010. 15061523, 2013. 2, pp. Shewanella algae is a gram-negative, rod-shaped, motile bacillus with a single polar flagellum. Bioprospeco da Chromobacterium violaceum para a biorremediao do chumbo: aplicaes em biotecnologia e educao em sade Shewanella putrefaciens has been described as a new aetiological agent of the disease, named shewanellosis, and could also be associated with different infections in humans, such as skin and tissue infections, bacteraemia, otitis. The majority of food infections have been related to the biofilm formation of foodborne pathogens in the food industry. However, reports of S. algae infection in aquatic animals are rare. First case of osteomyelitis due to Shewanella alga. S. algae is a facultative anaerobe, and has the ability to use metallic cations as electron acceptors in the electron transport chain if oxygen is not available[11]. Accessibility were extracted, 16S rDNA was PCR amplified and sequenced with universal 16S rDNA primers. DC16DC20, 2015. Cho, B. Kang et al., A case of spontaneous bacterial peritonitis with bacteremia caused by Shewanella algae, Infection & Chemotherapy, vol. 250255, 2009. Unable to load your collection due to an error, Unable to load your delegates due to an error. 168, no. Volume 63: 129-133. 229, no. There were several limitations in this study. 443448, 2000. Marine Drugs | Free Full-Text | Agarose-Degrading Characteristics of a Peer Review reports Background The genus Shewanella comprises members of Gram-negative, motile, oxidase-positive, facultative anaerobic and motile rods. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. Shewanella the environmentally versatile genome - Nature Would you like email updates of new search results? P.-Y. This study demonstrated the occurrence of putative pathogen S. algae in ballast water of ships docked at Malaysian port with positive reaction for cytochrome oxidase, catalase and high tolerance to NaCl. -. Therefore, these salt-tolerant bacteria are potential food-borne pathogens. 67, no. Promoted Reduction of Tellurite and Formation of Extracellular an important role in detoxication transforming the . (Km) and maximum velocity (Vmax) of pNPA-hydrolyzing reaction were 12.6 mM and 550 U.mg-1 . The flasks were inoculated with bacterial culture and incubated at 30C on a rotator shaker (180rpm) for 48hrs. Shewanella algae has been identified as a new bacterial species, Shewanella spp., from clinical samples [2]. Shewanella algae is of interest because of its potential to be a serious human pathogen. The aquaculture samples were placed in sterile plastic bags, and water samples were collected in transportation tubes. It can be isolated from a wide range of environments, including fresh water, estuary, and the deep sea [4]. The nucleotide was purified by using a QIAquick PCR purification kit (Qiagen). Dis, 1997. Pan-genome analyses of 24 Shewanella strains re-emphasize the Among these, the isolation rates of Shewanella algae were 2/18 in abalone (Haliotis diversicolor), 11/36 in oyster (Crassostrea angulata), and 6/30 in clams including Meretrix lusoria, Perna viridis, and Geloina erosa (Table 1). Shewanella species are distributed ubiquitously in the soil and water, being common in the marine habitat. 13, no. 18, no. R. Finkelstein and I. Oren, soft tissue infections caused by marine bacterial pathogens: epidemiology, diagnosis, and management, Current Infectious Disease Reports, vol. [11] Ogi, Takashi, et al. Clipboard, Search History, and several other advanced features are temporarily unavailable. S. algae strains from all isolates exhibited hemolysis on sheep blood agar. Therefore, the aim of this work was to study in vitro the biochemical reactions involving the TMAO-reduction by Shewanella baltica and the in fluence of acetate and C02 on this reaction. Comparing the two isolates of the curves based on optical density, the curves of growth rate versus temperature are in direct proportion as drawn in Figure 2. Phylum: Proteobacteria 188-189, 2014. Journal of Korean Medical Science, 2009. Shewanella species are highly advanced among . Hemolysis occurred in sheep blood agar after incubation at two different temperatures (25C and 37C) (Table 3). Shewanella M. Satomi, in Encyclopedia of Food Microbiology (Second Edition), 2014 Abstract Shewanella are Gram negative, motile rods with positive oxidase and catalase reactions. 13, pp. 37, no. Disclaimer, National Library of Medicine DNAs of Shewanella spp. [3]. The Basic Microbiology Curriculum: Biochemicals and Gram-Negative Organisms ID Course is a blended learning activity that includes both eLearning and handson laboratory exercises. W. Jiang, B. Xia, and Z. Liu, A serine hydroxymethyltransferase from marine bacterium Shewanella algae: isolation, purification, characterization and l-serine production, Microbiological Research, vol. Shewanella algae and Shewanella putrifaciens: clinical and microbiological characteristics. T. Wang, Model of life expectancy of chronic hepatitis B carriers in an endemic region, Journal of Epidemiology, vol. Other studies previously found that a few Shewanella species could utilize L-arabinose and glucose [9, 50]; however, we were not able to confirm these results. 102, no. These results all indicate that S. algae is capable of adapting to environmental changes. Careers. Researcher Profile - YAMADA Yasuyuki We collected 109 samples and identified S. algae in 23% (19/84) of isolates from shellfishes and in 28% (7/25) of water isolates (Tables 1 and 2). official website and that any information you provide is encrypted Essential for the anaerobic respiratory flexibility of shewanellae, CymA transfers electrons from menaquinol to various dedicated systems for the reduction of terminal electron acceptors including fumarate and insoluble minerals of Fe(III). Molecular weight of the enzyme was 62.3 kDa according to SDS-PAGE data. Shewanella algae are mesophilic organisms found in aquatic environments. However, this process can lead to undesirable nitrite accumulation and subsequent ammonium production. N. Vignier, R. Thodose, M. Barreau et al., Human infection with Shewanella putrefaciens and S. algae: report of 16 cases in martinique and review of the literature, American Journal of Tropical Medicine and Hygiene, vol. A. Martins et al., Photoprotective bioactivity present in a unique marine bacteria collection from Portuguese deep sea hydrothermal vents, Marine Drugs, vol.
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